Multidisciplinary Research Unit (MRU, ICMR), AMCH

Year of Initiation: 2012
Principal Investigator : Dr. Ratan Kumar Kotokey

Principal-cum-chief Superintendent, Assam Medical College
Co-ordinator : Dr. Lahari Saikia
Professor & Head, Dept. of Microbiology, Assam

Introduction

The Multidisciplinary Research Unit at Assam Medical College & Hospital, Dibrugarh, is a Govt. of India initiative established with Grants-in-aid received from the Indian Council of Medical Research (ICMR), New Delhi with a view to forward Bio-medical research in the region.
This is the first Laboratory of its type in the region established under the Central Govt. initiative ‘Establishment of Multi-Disciplinary Research Units in Government Medical colleges/Research Institutions’ and was formally inaugurated by Dr. V.M. Katoch, Secretary to Govt. of India, Dept. of Health Research (MH&FW) & Director General, ICMR, on the 11th of May, 2013 in the esteemed presence of Dr. Atindra Kr. Adhikari, Principal-cum-chief Superintendent, Assam Medical College & Hospital, Dibrugarh.


Divisions and Facilities

The Research Unit at Assam Medical College has three broad sections – Biochemistry, Pathology and Microbiology; each with dedicated working areas. In addition, there is a dedicated Molecular Biology section with separate Pre and Post amplification rooms. The laboratory is equipped with Autoanalysers, automated cell-counter, PCR (conventional and Real-time) stations, Elisa readers and washers, Anaerobic culture system, Histopathology equipments, Deep freezers, Bio-Safety Cabinets, western blotting and water purification system and dedicated media preparation and sterilisation rooms.


Activities

The laboratory is currently being used for conducting research in the field of clinical medicine and basic science. It supports both intramural and extramural projects carried out under different clinical researchers of the institute. In addition, the laboratory facilities are also being used by Post-graduate students for carrying out their thesis related work. The laboratory is also utilised for generating preliminary data about important health problems with a view to generate funds for large-scale projects in the future.


 
  Thermo Scientific Arktik Thermal Cycler Light Cycler 480 II (Roche)
 
  ELISA
 
 
Cobas c111 automated system (Roche)
Semi-automated analyser (VChem Next)
 
  ROUTINE BLOOD ANALYSIS HISTOPATHOLOGICAL EXAMINATION
 
  BIOBANKING ANAEROBIC/ MICROAEROPHILIC CULTURE
Available Research Facilities
  1. Polymerase Chain Reaction (PCR)
    • Conventional
    • Real-time (quantitative)
  2. Elisa
  3. Biochemical analysis
  4. Routine Blood examination
  5. Histo-pathological examination
  6. Biobanking
  7. Anaerobic/Microaerophilic culture





Projects

Completed Projects

  • Assessment of oxidative stress correlating the levels of antioxidant enzymes and non-dietary antioxidants with extent of lipid peroxidation in drug naïve Schizophrenia patients in Assam. (Intramural Project)
  • Sero-surveillance of Taenia solium IgG antibodies in Acute Encephalitis Syndrome cases. (Intramural Project)
  • Occurrence of Respiratory Syncytial virus, Influenza A and B virus, Parainfluenza 1, 2, 3 virus and Human Metapneumovirus in Acute Respiratory Tract Infection in paediatric patients – A hospital based study. (Intramural Project)
  • Spectrum of bacterial pathogens causing Community Acquired Pneumonia in children under 5 years of age. (Intramural Project)
  • Evaluation of the virulence factors of Candida albicans isolated from HIV positive patients. (Intramural Project)

Ongoing Projects

  • Association of Helicobacter pylori virulence factors with upper gastrointestinal and extra-intestinal diseases in adults.
  • A clinical and serological study of childhood neurocysticercosis with suggestive lesion on CT scan brain.
  • Isolation and characterization of Helicobacter pylori from gastric biopsy specimen.
  • Blood zinc levels in children with severe Pneumonia and effects of zinc supplementation. (Extramural Project – ICMR)

 

Approved Projects to be Initiated Shortly

  • A study of comparative evaluation of conventional and molecular method for detection of childhood bacterial pneumonia - a hospital based study.
  • Characterization of virulence markers in shigella species and its correlation with disease manifestations in patients with diarrhea and dysentery.
  • Microbial etiology of empyema thoracis along with risk factor analysis in a tertiary care hospital in Assam.
  • Role of MR-spectroscopy, Fractional anisotropy and ADC values in Minimal Hepatic Encephalopathy: correlating with serum pro-inflammatory cytokines and neuro-psychiatric assessment.

 

Projects Submitted for Approval

  • Identification of deregulated molecular patterns in biofluids of Japanese encephalitis patients to predict early clinical phenotypes.
  • Aetiology of childhood pneumonia in North-East India and effect of viral and bacterial co-infection in disease progression and outcome.
  • Cerebrospinal fluid in Tubercular meningitis:: a study of deregulated molecular metabolic profile.

 

Number and categories of personnel in the laboratory
    The following personnel are employed on a contractual basis for the entire laboratory as per the MRU establishment guidelines:
  • Research Scientist II: Dr. Saurav Jyoti Patgiri, M.D. Microbiology.
  • Research Scientist I: Dr. Himangshu Mazumdar, M.D. Biochemistry.
  • Laboratory technicians: Ms. Soni Begum.

Workshops and Trainings
    CME cum Workshop
  • A 3 day CME cum Workshop including hands on training was conducted on “Molecular Diagnostic Methodology” in the month of November ‘2013 among the faculty members and post graduate students of different medical colleges of Assam.
    Date: 05/11/2013 to 07/11/2013
    Venue: Multi-disciplinary Research Laboratory, AMCH
    Total no. of participants: 24
    Participating Medical Colleges: Assam Medical College & Gauhati Medical College

  • On-site Training on Western blotting: Date: 20.10.2014 to 25.10.2014
    Venue: Multi-disciplinary Research Laboratory, AMCH
    Total no. of participants: 8
    Conducted by: Dr. R.K.Nanda, Staff research Scientist, ICGEB, New Delhi.